Quality improvement lessons from Canadian thyroid and parathyroid surgery legal decisions.

BACKGROUND: This is the first study of Canadian thyroid and parathyroid surgery legal decisions, and the first study of surgical malpractice using the Canadian Legal Information Institute (CanLII) database. The objective was to identify quality improvement opportunities in surgical practice, to increase patient safety and satisfaction. METHODS: Legal decisions relating to thyroid and parathyroid surgery in the CanLII database were screened. Cases were included if a surgeon was listed as applicant or respondent; they related to pre-, intra-, or post-operative management of thyroid or parathyroid disease; and malpractice was alleged. Cases were excluded if surgery was mentioned incidentally or for non-surgical focus. RESULTS: Of the 347 unique legal decisions screened, 14 met inclusion and exclusion criteria. Surgeries occurred between 1976 and 2012, with 13 thyroid surgeries, 1 parathyroidectomy, and 4 mortalities. CONCLUSIONS: Quality improvement lessons include communication, pre-operative patient education and documentation of risks discussed, and in-person assessment of complications.

Frailty as a Predictor of Adverse Outcomes After Peripheral Vascular Surgery in Patients With End-Stage Renal Disease.

BACKGROUND: Frailty and end-stage renal disease (ESRD) are each independently associated with adverse outcomes in patients who undergo lower extremity vascular bypass operations (LEOs). It is not known whether frailty imparts additional risk to patients with ESRD having LEO. METHODS: The study was a retrospective cohort study of 29 203 patients without ESRD and 1718 with ESRD who had LEO surgery between the years 2014 and 2018 extracted from the American College of Surgeons National Surgical Quality Improvement Program database. Preoperative frailty was assessed using a simplified frailty index based on a history of diabetes, heart failure, chronic obstructive pulmonary disease, hypertension, and functional status. Adverse outcomes measured were 30-day mortality, surgical complications, reoperation, length of stay, readmission, discharge destination, and any ("composite") adverse outcome. Odds ratios (ORs) for adverse outcomes were calculated with logistic regression. RESULTS: Among patients with ESRD, the OR for having the composite adverse outcome was greater for frailty than for any of the other preoperative risk factors studied (OR 2.191, CI 1.569-3.061, P < .001). Adverse outcomes occurred in 84.3% of frail patients with ESRD, but in only 39.6% of the non-frail patients without ESRD. CONCLUSIONS: Frailty imparts additional risk for adverse outcomes to patients with ESRD undergoing LEO.

Orthomageddon: A retrospective cohort study of weather-dependent variations in emergency department volume in a Canadian city.

INTRODUCTION: Unique weather patterns can dramatically increase the number of emergency department (ED) visits due to falls on snow or ice compared to winter averages. They can create "Orthomageddon" incidences; days when the number of orthopedic injuries dramatically exceeds average. The study objective was to identify weather-dependent differences in demographics, length-of-stay (LOS) predictors, and volume for fall-injury presenting to the ED. The authors placed emphasis on Chinook or Foehn phenomenon (rapid freeze-thaw cycles) common east of the Rocky Mountains. METHOD: Patients with extremity and hip fractures from fall on snow or ice were identified from the Calgary Zone Alberta Health Services ED database from November 1st 2013 to March 31st 2018 (n = 3894). High-volume dates were the 90th percentile of all dates by volume (n = 76). This equated to all dates with >10 fall-injuries. The authors compared post-Chinook, night-freezing, high-volume, and regular winter conditions. Meteorological data was collected from the Environment Canada weather station at the Calgary International Airport. RESULTS: The authors identified 588 post-Chinook, and 1149 night-freezing presenters. Weather was strongly predictive of ED fall-injury volume. Night-freezing events (above-freezing temperatures prior to 1800 hours the preceding day, followed by freezing temperatures prior to 0600 hours the following day) correlated with elevated fall-injury volume (OR, 6.84; 95% CI, 5.88, 7.97) as were recent Chinook events (OR, 2.19; 95% CI, 1.84, 2.62). CONCLUSION: Weather, particularly nighttime conditions, are highly predictive of winter, fall-related ED volume. This may inform future population-level alerts for dates of elevated fall risk and ED staffing patterns.

Reduced Uteroplacental Perfusion Pressure (RUPP) causes altered trophoblast differentiation and pericyte reduction in the mouse placenta labyrinth.

This study characterized the effect of the reduced utero-placental perfusion pressure (RUPP) model of placental insufficiency on placental morphology and trophoblast differentiation at mid-late gestation (E14.5). Altered trophoblast proliferation, reduced syncytiotrophoblast gene expression, increased numbers of sinusoidal trophoblast giant cells, decreased Vegfa and decreased pericyte presence in the labyrinth were observed in addition to changes in maternal blood spaces, the fetal capillary network and reduced fetal weight. Further, the junctional zone was characterized by reduced spongiotrophoblast and glycogen trophoblast with increased trophoblast giant cells. Increased Hif-1α and TGF-ß-3 in vivo with supporting hypoxia studies in trophoblast stem (TS) cells in vitro, support hypoxia as a contributing factor to the RUPP placenta phenotype. Together, this study identifies altered cell populations within the placenta that may contribute to the phenotype, and thus support the use of RUPP in the mouse as a model of placenta insufficiency. As such, this model in the mouse provides a valuable tool for understanding the phenotypes resulting from genetic manipulation of isolated cell populations to further understand the etiology of placenta insufficiency and fetal growth restriction. Further this study identifies a novel relationship between placental insufficiency and pericyte depletion in the labyrinth layer.

Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta.

Trophoblast stem (TS) cells in the mouse derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5) to support placental development. Further development and growth is proposed to rely on layer-restricted progenitor cells. Stem cell antigen (Sca) -1 is a member of the Ly6 gene family and a known marker of stem cells in both hematopoietic and non-hematopoietic mouse tissues. Having identified that Sca-1 mRNA was highly expressed in mouse TS cells in culture, we found that it was also expressed in a subset of trophoblast within the chorion and labyrinth layer of the mouse placenta. Isolation and in vitro culture of Sca-1+ trophoblast cells from both differentiated TS cell cultures and dissected mouse placentae resulted in proliferating colonies that expressed known markers of TS cells. Furthermore, these cells could be stimulated to differentiate and expressed markers of both junctional zone and labyrinth trophoblast subtypes in a manner comparable to established mouse TS cell lines. Our results suggest that we have identified a subpopulation of TS cell-like cells that persist in the mid- to late- gestation mouse placenta as well as a cell surface protein that can be used to identify and isolate these cells.